Abstract: In order to understand the influence of temperature on dissolution rate in Ordovician carbonate brock in different water flow rate, dissolution experiment was conducted at different temperature, different water flow rate and different CO₂ pressure with self-designed and manufactured dissolution device which can simulates water flow conditions and contains 24 samples as platform, samples collected from Zhangji mine in Huainan, Zhangcun mine in Luan and Dongtan mine in Yanzhou were used as representative samples. The experiment results show that when water flow rate is relatively low(16.67 mL/min), the influence of environmental temperature change on dissolution rate is insignificant(the average dissolution volume is 0.0006g/cm²), when the water flow rate is high (60mL/min), the influence of temperature change on dissolution rate is significant (the average dissolution volume is 0.0038g/cm²). That is to say, in strong run-off zone of ground water, the influence of temperature on dissolution rate is bigger and the dissolution rate is higher, so karst is developed.