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露天煤矿排土场土层重构及接菌对植物根系提水作用试验研究

毕银丽 高学江 柯增鸣 肖礼

毕银丽,高学江,柯增鸣,等. 露天煤矿排土场土层重构及接菌对植物根系提水作用试验研究[J]. 煤田地质与勘探,2022,50(12):12−20 doi: 10.12363/issn.1001-1986.22.07.0595
引用本文: 毕银丽,高学江,柯增鸣,等. 露天煤矿排土场土层重构及接菌对植物根系提水作用试验研究[J]. 煤田地质与勘探,2022,50(12):12−20 doi: 10.12363/issn.1001-1986.22.07.0595
BI Yinli,GAO Xuejiang,KE Zengming,et al. Experimental study on effect of soil layer reconstruction and inoculation on plant root hydraulic lift in open-pit coal mine dump[J]. Coal Geology & Exploration,2022,50(12):12−20 doi: 10.12363/issn.1001-1986.22.07.0595
Citation: BI Yinli,GAO Xuejiang,KE Zengming,et al. Experimental study on effect of soil layer reconstruction and inoculation on plant root hydraulic lift in open-pit coal mine dump[J]. Coal Geology & Exploration,2022,50(12):12−20 doi: 10.12363/issn.1001-1986.22.07.0595

露天煤矿排土场土层重构及接菌对植物根系提水作用试验研究

doi: 10.12363/issn.1001-1986.22.07.0595
基金项目: 国家重点研发计划项目(2022YFF1303300);国家自然科学基金面上项目(51974326)
详细信息
    第一作者:

    毕银丽,1971年生,女,陕西米脂人,博士,长江学者特聘教授,博士生导师,从事矿山生态修复研究. E-mail:ylbi88@126.com

  • 中图分类号: TD824.8;P642.16

Experimental study on effect of soil layer reconstruction and inoculation on plant root hydraulic lift in open-pit coal mine dump

  • 摘要: 露天煤矿排土场土层重构对于生态重建具有重要意义,为研究接种深色有隔内生真菌(dark septate endophytes, DSE)对不同重构土层模式下玉米根系水分的利用效应,采用土柱模拟培养试验,设置4种类型土层处理,每种土层类型下设置接菌及对照处理,共8组处理。结果表明:掺黄土20%处理下玉米根长密度最大,分别为掺黄土0%、10%和40%的3.2、2.4、2.8倍,水分胁迫后根系具有向下生长、吸取深层水分的能力;基于δ18O值的MixSIAR模型水源分析,掺黄土0%处理下玉米主要利用0~25 cm处的水分,水分利用效率达到80%;掺黄土10 %处理下玉米主要利用15~35 cm处的水分,水分利用效率达到64%;而掺黄土20%处理下玉米对0~25 cm处的水分利用效率仅为36%,对25~45 cm处水分利用率达到64%,说明掺黄土20%处理下玉米主要利用土壤深层水分。接种DSE提高了植物吸收更深层水的能力,掺黄土20%处理下水分利用深度向下增加了5 cm,掺黄土20%基质中接菌处理在干旱胁迫后植物根系提水量达到最大,生长期总提水量较不接菌处理提升了45%;在不接菌条件下掺黄土20%植物根系提水量是掺黄土0%的1.45倍,而在接菌条件下掺黄土20%植物根系提水量可达到掺黄土0%的1.72倍。综上认为,接种DSE及土层重构均对提升植物提水能力具有显著作用。此外,本研究结果对露天矿区排土场土层重构过程中土壤改良及植物的水分利用效率提供实验参考依据。

     

  • 图  土柱试验装置

    Fig. 1  Soil column test device

    图  不同处理各层的根质量密度和根长密度

    Fig. 2  Root mass density and root length density in each layer of different treatments

    图  不同处理土壤最终含水率

    Fig. 3  Final soil moisture content for different treatments

    图  不同处理玉米水、土壤水中δ18O的变化

    Fig. 4  Changes of δ18O in corn water and soil water under different treatments

    图  不同处理玉米对各层水分利用比例

    Fig. 5  The water utilization ratio of different maize treatments to each layer

    图  玉米水分胁迫下提水量

    Fig. 6  The amount of water to be lifted under water stress in maize

    表  1  土壤颗粒机械组成

    Table  1  Soil particle size compositions

    土质
    类型
    各粒径质量分数/%
    黏粒
    (<0.002 mm)
    粉粒
    (0.002~0.020 mm)
    砂粒
    (>0.020 mm)
    S00.62b22.53c72.50a
    S10.67b25.42b70.16ab
    S20.76b27.16b68.26b
    S31.35a36.93a60.48c
      注:同列数字后的字母不相同表示0.05水平上差异显著。
    下载: 导出CSV

    表  2  玉米地上部分各指标统计

    Table  2  Statistical table of each index of maize above ground

    处理方式株高/cm茎粗/cm鲜重/g干重/g全氮质量分数/%全磷质量分数/%侵染率/%
    S0+M81±6.0c6±0.52cd16.6±1.2d2.5±0.08c1.8±0.06d0.69±0.02d42.46
    S0−M70±4.9d5.3±0.17d7.0±0.8e1.2±0.06d1.1±0.04e0.57±0.01e
    S1+M112±7.2b7.2±0.93ab29.8±1.2ab4.5±0.11b3.6±0.09ab0.81±0.02bc26.50
    S1−M105±6.5b6.9±0.65bc26.7±1.3b4.2±0.09b3.5±0.12bc0.77±0.02c
    S2+M124±4.0a8.3±0.47a38.0±1.8a6.5±0.15a4.1±0.15a0.86±0.04a28.47
    S2−M115±2.1ab8.2±0.25a32.6±1.6ab6.2±0.20a4.1±0.10a0.86±0.02a
    S3+M111±6.8b8.1±0.87a34.0±1.4ab5.3±0.15ab3.6±0.08ab0.82±0.03ab25.40
    S3−M109±4.5b7.8±0.22ab31.2±1.5ab4.6±0.13b3.0±0.06c0.81±0.01bc
    S******************
    M**Ns*******
    SMNsNsNsNsNs**
      注:S代表重构土层;M代表接菌;Ns代表显著性水平P≥0.05;*代表P<0.05;**代表 P<0.01;***代表 P<0.001;同列的字母不相同表示0.05水平上差异显著。
    下载: 导出CSV

    表  3  不同处理各层3个等级根的根长密度百分比统计

    Table  3  Statistical table of root length density percentage of three grades of roots in different treatments

    根类型深度/cm根长密度百分比/%
    S0+MS0−MS1+MS1−MS2+MS2−MS3+MS3−M
    三级根0~155857514655544648
    15~255256556062616054
    25~355655606066635250
    35~45520583959596259
    次根0~152425262924253226
    15~252629292521212736
    25~352932262720233044
    35~45320244425242730
    主根0~151818232521212226
    15~252215161517181310
    25~35151314131414186
    35~45160181716171111
    下载: 导出CSV
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  • 收稿日期:  2022-07-30
  • 修回日期:  2022-09-21
  • 刊出日期:  2022-12-25
  • 网络出版日期:  2022-12-02

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