Micro CT-based meticulous characterization of porosity evolution of coal in the process of biodegradation
-
摘要: 为了查明产甲烷菌群对不同变质程度煤厌氧发酵所产生的生物增透效应，利用显微CT对生物降解前后的煤样进行三维重建，分析煤的孔隙结构变化特征，通过扫描电镜观察产甲烷菌群在煤表面的吸附特征，探讨不同煤阶煤生物改造效果差异的原因。实验结果发现，厌氧发酵后煤样的孔隙率和吼道总长度增加，孔隙的连通性增强，表明在厌氧发酵过程中煤被微生物降解，促进煤中新孔隙的形成、扩展和贯通，从而实现煤的生物增透效应。随着煤的变质程度降低，煤中孔隙结构明显改善，主要原因在于产甲烷菌群更倾向于吸附在低阶煤的表面。研究结果可为煤层气生物工程的现场应用提供理论指导。Abstract: To explore the bio-permeability enhancing effect via methanogen treatment on different rank coals after anaerobic fermentation, micro-CT was used to analyze the change characteristics of pore structure of coal before and after biodegradation. At the same time, the adsorption characteristics of methanogens on the coal surface after anaerobic biodegradation were observed by scanning electron microscopy to analyze the reasons for the effect difference of biological transformation of different rank coals. The results show that the porosity and total throat length of the coal samples biodegraded increase and the connectivity of the pores is enhanced, it is indicated that coal samples were degraded by microorganisms, which promotes the formation, expansion and penetration of new pore in the coal samples, thus realizing the bio-permeability enhancing effect of coal. With the decrease of coal rank, the improvement of pore structure is more obvious. The main reason for the change is that the surface of low rank coal is more suitable for the adsorption of methanogens. The research can provide a theoretical reference for the domestic coalbed biogenic gas production.
-
Keywords:
- coal /
- anaerobic fermentation /
- pore structure /
- micro-CT /
- methanogens
-
-
[1] MAYUMI D,MOCHIMARU H,KAMAGATA Y,et al. Methane production from coal by a single methanogen[J]. Science,2016,354(6309):222-225.
[2] DARIUSZ S,MASTALERZ M,DAWSON K,et al. Biogeochemistry of microbial coalbed methane[J]. Annual Review of Earth & Planetary Sciences,2011,39(39):617-656.
[3] SCOTT A R. Thermogenic and secondary biogenic gases,San Juan basin,Colorado and New Mexico-implications for coalbed gas producibility[J]. American Association of Petroleum Geologists Bulletin,1994,78(8):1186-1209.
[4] RITTER D,VINSON D,BARNHART E,et al. Enhanced microbial coalbed methane generation:A review of research,commercial activity,and remaining challenges[J]. International Journal of Coal Geology,2015,146:28-41.
[5] 孙斌,李金珊,承磊,等. 低阶煤生物采气可行性:以二连盆地吉尔嘎朗图凹陷为例[J]. 石油学报,2018,39(11):1272-1278. SUN Bin,LI Jinshan,CHENG Lei,et al. The feasibility of biological gas recovery in low-rank coal:A case study of Jiergalangtu depression in Erlian basin[J]. Acta Petrolei Sinica,2018,39(11):1272-1278.
[6] 白超峰,岳前升,吴洪特,等. BCTG生物煤层气技术与发展前景[J]. 中外能源,2014,19(8):25-29. BAI Chaofeng,YUE Qiansheng,WU Hongte,et al. Biogenic coal-to-gas bio coalbed methane technology and development prospects[J]. Sino-Global Energy,2014,19(8):25-29.
[7] 任付平,韩长胜,王玲欣,等. 微生物提高煤层气井单井产量技术研究与实践[J]. 石油钻采工艺,2016,38(3):395-399. REN Fuping,HAN Changsheng,WANG Lingxin,et al. Microbially enhanced CBM well production rate technology and its application[J]. Oil Drilling & Production Technology,2016,38(3):395-399.
[8] OREM W H,VOYTEK M A,JONES E J,et al. Organic intermediates in the anaerobic biodegradation of coal to methane under laboratory conditions[J]. Organic Geochem-istry,2010,41(9):997-1000.
[9] FERRY J G. Fundamentals of methanogenic pathways that are key to the biomethanation of complex biomass[J]. Current Opinion in Biotechnology,2011,22(3):351-357.
[10] 夏大平,陈鑫,苏现波,等. 氧化还原电位对低煤阶煤生物甲烷生成的影响[J]. 天然气工业,2012,32(11):107-110. XIA Daping,CHEN Xin,SU Xianbo,et al. Impact of oxidation-reduction potential on the generation of biogenic methane in low-rank coals[J]. Natural Gas Industry,2012,32(11):107-110.
[11] ÜNAL B,PERRY V R,SHETH M,et al. Trace elements affect methanogenic activity and diversity in enrichments from subsurface coalbed produced water[J]. Frontiers in Microbiology,2012,3:175.
[12] 苏现波,徐影,吴昱,等. 盐度、pH对低煤阶煤层生物甲烷生成的影响[J]. 煤炭学报,2011,36(8):1302-1306. SU Xianbo,XU Ying,WU Yu,et al. Effect of salinity and pH on biogenic methane production of low rank coal[J]. Journal of China Coal Society,2011,36(8):1302-1306.
[13] 王爱宽,秦勇. 生物成因煤层气实验研究现状与进展[J]. 煤田地质与勘探,2010,38(5):23-27. WANG Aikuan,QIN Yong. Research status and progress of experimental study on biogenic coalbed methane[J]. Coal Geology & Exploration,2010,38(5):23-27.
[14] 陈浩,李贵中,陈振宏,等. 微生物提高煤层气产量模拟实验研究[J]. 煤田地质与勘探,2016,44(4):64-68. CHEN Hao,LI Guizhong,CHEN Zhenhong,et al. Simulation experiment on enhancing coalbed methane production by microbes[J]. Coal Geology & Exploration,2016,44(4):64-68.
[15] 郭红玉,苏现波,夏大平,等. 一种煤储层的生物增透方法:ZL201210417802.3[P]. 2015. [16] GUO Hongyu,DONG Zhiwei,SU Xianbo,et al. Synergistic biodegradation of coal combined with corn straw as a sub-strate to methane and the prospects for its application[J]. Energy & Fuels,2018,32(6):7011-7016.
[17] YAO Yanbin,LIU Dameng,CHE Yao,et al. Non-destructive characterization of coal samples from China using micro focus X-ray computed tomography[J]. International Journal of Coal Geology,2009,80(2):113-123.
[18] YAO Yanbin,LIU Dameng,CAI Yidong,et al. Advanced characterization of pores and fractures in coals by nuclear magnetic resonance and X-ray computed tomography[J]. Science in China Series D:Earth Sciences,2010,53(6):854-862.
[19] 苟启洋,徐尚,郝芳,等. 纳米CT页岩孔隙结构表征方法:以JY-1井为例[J]. 石油学报,2018,39(11):1253-1261. GOU Qiyang,XU Shang,HAO Fang,et al. Characterization method of shale pore structure based on nano-CT:A case study of well JY-1[J]. Acta Petrolei Sinica,2018,39(11):1253-1261.
[20] 张瑞玲,隋红,李洪,等. 微生物在土壤中迁移物化参数的确定[J]. 环境科学,2011,32(3):901-907. ZHANG Ruiling,SUI Hong,LI Hong,et al. Determination the parameters of bacteria transport in soil[J]. Environmental Science,2011,32(3):901-907.
[21] VAN LOOSDRECHT M C M,NORDE W,LYKLEMA J,et al. Hydrophobic and electrostatic parameters in bacterial adhesion[J]. Aquatic Sciences,1990,52(1):103-114.
[22] 冯雅丽,李浩然,连静,等. 利用微生物电池研究微生物在矿物表面电子传递过程[J]. 工程科学学报,2006,28(11):1009-1013. FENG Yali,LI Haoran,LIAN Jing,et al. Study on the electron transport process of microbe on the mineral surface using the microbe fuel cell[J]. Chinese Journal of Engineering,2006,28(11):1009-1013.
[23] FLEMINGER G,SHABTAI Y. Direct and rapid analysis of the adhesion of bacteria to solid surfaces:Interaction of fluorescently labeled rhodococcus strain GIN-1(NCIMB 40340) cells with titanium-rich particles[J]. Applied & Environmental Microbiology,1995,61(12):4357-4361.
计量
- 文章访问数: 239
- HTML全文浏览量: 22
- PDF下载量: 28
下载:
